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2-BBB started in 2003 as to-BBB technologies BV, a spin-off company from the renowned Blood-Brain Barrier Research Group of the Leiden Academic Center for Drug Research (LACDR) from Leiden University in the Netherlands. The founding fathers of to-BBB were Pieter Gaillard, Alfred Nijkerk (VenGen), Bert de Boer (emeritus head of the LACDR Blood-Brain Barrier Research Group) and Leiden University.
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